New Avirulence Genes in the Phytopathogenic Fungus Leptosphaeria maculans

Analysis of two pathogenicity genes of the blackleg fungus Leptosphaeria maculans

Rapid identification of the Leptosphaeria maculans avirulence gene AvrLm2 using an intraspecific comparative genomics approach.

Five avirulence genes from Leptosphaeria maculans, the causal agent of blackleg of canola (Brassica napus), have been identified previously through map-based cloning. In this study, a comparative genomic approach was used to clone the previously mapped AvrLm2. Given the lack of a presence-absence gene polymorphism coincident with the AvrLm2 phenotype, 36 L. maculans isolates were resequenced an...

Epigenetic Control of Effector Gene Expression in the Plant Pathogenic Fungus Leptosphaeria maculans

Plant pathogens secrete an arsenal of small secreted proteins (SSPs) acting as effectors that modulate host immunity to facilitate infection. SSP-encoding genes are often located in particular genomic environments and show waves of concerted expression at diverse stages of plant infection. To date, little is known about the regulation of their expression. The genome of the Ascomycete Leptosphae...

Isocitrate lyase is essential for pathogenicity of the fungus Leptosphaeria maculans to canola (Brassica napus).

A pathogenicity gene has been identified in Leptosphaeria maculans, the ascomycetous fungus that causes blackleg disease of canola (Brassica napus). This gene encodes isocitrate lyase, a component of the glyoxylate cycle, and is essential for the successful colonization of B. napus. It was identified by a reverse genetics approach whereby a plasmid conferring hygromycin resistance was inserted ...

Identification of the corresponding avirulence gene of Leptosphaeria maculans to the resistance gene (LepR1) in Brassica napus

Blackleg of canola is the most important disease of canola/rapeseed worldwide. Efforts are made to understand the host-pathogen interaction by studying the interaction between resistance genes of the host and the avirulence genes of the pathogen. Previous studies showed that two double haploid canola (Brassica napus L.) lines, ddm-12-65-1 and ddm-12-65-2, possess a novel resistance gene (LepR1)...